Elveflow microfluidic system for dynamic cell culture

France Elveflow focuses on the development and manufacturing of microfluidic instruments and systems, with multi-channel OB1 pressure controller as the main equipment, combined with MFS flow sensor, MPS pressure sensor, MUX recirculation circulation valve, A complete set of microfluidic application systems was constructed, among which the microfluidic dynamic cell culture system and the microfluidic cell perfusion culture system were used as microfluidic systems for cell culture.

Microfluidic cell culture is a dynamic cell culture in a microfluidic chip using a microfluidic system. The size of the microfluidic cell culture chip is suitable for the size of the cell, so it is used in dynamic cell culture. The key to microfluidic cell culture is the ability to simulate the cell microenvironment. The advantages of using microfluidic cell culture include increasing the physiological relevance of the microenvironment, reducing the scale to the scale of tissues, creating complex co-culture systems, applying dynamic and mechanical forces, increasing the level of tissue interface simulation, and reducing animal experimental models for drug testing. Simulate the flow of blood, lymph and interstitial fluid.

 Elveflow uses microfluidic system for cell culture application include:

· Study on cell interaction on microfluidic chips

· A microfluidic system was used to study the adaptability of bacteria to pressure and environmental changes

· Recirculation of cell culture media using microfluidic circulation valves

· Cell culture cycle in dynamic cell culture

· How to do cell staining for dynamic cell culture in microfluidic chips

· Cell culture medium perfusion in microfluidic dynamic cell culture

· Automatic cell inoculation in microfluidic dynamic cell culture

· Microfluidic cardiomyocyte culture model

· The microfluidic cell culture perfusion system was used for rapid cell medium switching

· For fluorescent reading of microfluidic qPCR

· Microfluidic system was used to capture circulating tumor cells

· Live cell detection on microfluidic chip

· Quantification of weak protein binding by microfluidic pressure pump

https://www.elveflow.com/microfluidic-application-notes/microfluidic-cell-culture/

Parameters of OB1 multi-channel pressure pump used in microfluidic cell culture:

OB1 Microfluidic pressure and flow controllers can be selected from 1-4 channels or more, the pressure and vacuum range of each channel is optional, 5 optional ranges are: 0-200mbar, 0-2000mbar, 0-8000mbar, -900-1000mbar, -900mbar-6000mbar.  It is modular and upgradeable, and is controlled by Elveflow ESI software.

OB1 Microfluidic Pressure and flow controller Performance parameters:

· Pressure stability 0.005% FS

· Corresponding time 9ms

· Pressure resolution 0.003%FS

· Stability time is less than 35ms

OB1 microfluidic pressure and flow controllers can be used for digital microfluidic, flow chemistry & polymer synthesis, cell perfusion in cell culture, sequential injection of cell culture solution, single drop sequencing, RNA sequencing, lab-on-a-chip, organ-on-a-chip and other applications.