Elveflow single-cell encapsulated microfluidic systems

Single-cell encapsulation of microdroplets is a technology that uses a microfluidic system to generate microdroplets for single-cell encapsulation. The microfluidic device driven by a pressure controller can produce droplets with larger skin size, which can be encapsulated and further analyzed at the single-cell level. Elveflow offers a complete set of microfluidic single-drop encapsulation instruments and related user manuals for single-cell encapsulation and single-cell sequencing of microdrops.

Applications for microfluidic single-cell encapsulation include:

Oncology (Insight into mutations carried by small cell subpopulations)

Immunology (distinguishing different immune cell populations or characterizing immune cell populations)

Neurology (Obtaining detailed single-cell maps to understand and identify the different types of neurons in the brain and their connecting molecules)

Developmental biology (mapping the entire development of an embryo cell by cell

Monocytomics (revealing cellular heterogeneity in tissue samples

The instrument configuration of Elveflow for single-cell encapsulation with microdroplets is as follows:

1.OB1 pressure controller with at least two 0-2000 mbar channels

2.1 xMFS2 Flow sensor, 0.4-7 μL/min, for oil phase solutions

3.1 Xmfs3 Flow sensor 4.2-80 μL/min for cell suspension

4.1x kit Luer lock starter connector

5.2 x 15 mL liquid storage tube

6. Microfluidic chip with hydrophobic channel

7. A microscope for observation

8. Camera for imaging (optional)

9.HF5-7500 + 1% FluoSurf surfactant

10.Aquapel for hydrophobic treatment (optional)

Microfluidic chip is a key component of microdroplet single-cell encapsulation. Flow-focused microfluidic microdroplet can be used to generate the chip, and the material is PDMS. The microchannel can be processed with Aquapel to form a hydrophobic microfluidic channel.

Fluidic 947 microdroplet generation chip can obtain 10-30μm droplets.

Fludic 440 microdroplet generation chip, can obtain 50-80μm droplets

A single cell encloses a microfluidic chip, generating a drop of 20-50 μm.

These chips have 2 inlets and 1 outlet, which are cell suspension and oil phase respectively, enabling single-cell encapsulation.

If single-cell post-encapsulation sequencing is desired, a microfluidic chip with 4 inlets and 1 outlet can be used, with 4 inlets for cell suspension, Barcoded hydrogel beads, lysis buffer and oil phase respectively.

For example, using a cell solution of 13.57X105 cells /ml, the mean diameter of the microdroplet was 51.2μm (CV=2.4%).